The LacdiNAc-Specific Adhesin LabA Mediates Adhesion of Helicobacter pylori to Human Gastric Mucosa

Archive ouverte : Article de revue

Rossez, Yannick | Gosset, Pierre | Boneca, Ivo G. | Magalhães, Ana | Ecobichon, Chantal | Reis, Celso A. | Cieniewski, Caroline | Joncquel, Marie | Leonard, Renaud | Maes, Emmanuel | Sperandio, Brice | Slomianny, Christian | Sansonetti, Philippe | Michalski, Jean-Claude | Robbe, Catherine

Edité par HAL CCSD ; Oxford University Press (OUP)

Adhesion of Helicobacter pylori to the gastric mucosa is a necessary prerequisite for the pathogenesis of H. pylori–related diseases. In this study, we investigated the GalNAcβ1-4GlcNAc motif (also known as N,N’-diacetyllactosediamine [lacdiNAc]) carried by MUC5AC gastric mucins as the target for bacterial binding to the human gastric mucosa. The expression of LacdiNAc carried by gastric mucins was correlated with H. pylori localization, and all strains tested adhered significantly to this motif. Proteomic analysis and mutant construction allowed the identification of a yet uncharacterized bacterial adhesin, LabA, which specifically recognizes lacdiNAc. These findings unravel a target of adhesion for H. pylori in addition to moieties recognized by the well-characterized adhesins BabA and SabA. Localization of the LabA target, restricted to the gastric mucosa, suggests a plausible explanation for the tissue tropism of these bacteria. These results pave the way for the development of alternative strategies against H. pylori infection, using adherence inhibitors.

Consulter en ligne

Suggestions

Du même auteur

Early detection of colonic dysplasia by magnetic resonance molecular imagin...

Archive ouverte: Article de revue

Rossez, Yannick | 2016-05-01

Human gastric mucin MUC5AC is secreted in the colonic mucus of cancer patients and is a specific marker of precancerous lesions called aberrant crypt foci. Using MUC5AC as a specific marker can improve sensitivity in the detection...

How do they stick together? Bacterial adhesins implicated in the binding of...

Archive ouverte: Article de revue

Ringot-Destrez, Bélinda | 2017-04-15

The gastrointestinal mucosal surface is the primary interface between internal host tissues and the vast microbiota. Mucins, key components of mucus, are high-molecular-weight glycoproteins characterized by the presence of many O-...

Interaction between DMBT1 and galectin 3 is modulated by the structure of t...

Archive ouverte: Article de revue

Rossez, Yannick | 2011-03

International audience. DMBT1 (deleted in malignant brain tumor 1), a human mucin-like glycoprotein, belonging to the scavenger receptor cystein-rich (SRCR) superfamily, is mainly secreted from mucosal epithelia. It...

Du même sujet

How do they stick together? Bacterial adhesins implicated in the binding of...

Archive ouverte: Article de revue

Ringot-Destrez, Bélinda | 2017-04-15

The gastrointestinal mucosal surface is the primary interface between internal host tissues and the vast microbiota. Mucins, key components of mucus, are high-molecular-weight glycoproteins characterized by the presence of many O-...

Droits de l'homme et libertés fondamentales / Henri Oberdorff,...

Livre | Oberdorff, Henri (1947-....). Auteur | 2023 - 9e édition

Les droits de l'homme et les libertés fondamentales constituent l'un des fondements essentiels de toute société démocratique. Ils représentent le patrimoine juridique commun de l'humanité compte tenu de leur universalisme. Le droi...

L' intelligence de l'animal / Jacques Vauclair

Livre | Vauclair, Jacques (1947-....). Auteur. Auteur de la postface, du colophon, etc. | 2017

La quatrième couverture indique : "Les amis des chiens ou des chats, comme ceux qui observent dans la nature oiseaux, insectes ou éléphants, sont persuadés que les animaux manifestent souvent des comportements " intelligents ". Le...

Atlas de biologie animale / Sandrine Heusser, Henri-Gabriel Dupuy

Livre | Heusser, Sandrine. Auteur | 2015

La quatrième de couverture indique : "Cet atlas offre une vision synthétique et illustrée des plans d'organisation et de la physiologie des animaux. Outre quelques eucaryotes unicellulaires, les grands groupes de Métazoaires, les ...

Que diraient les animaux, si... on leur posait les bonnes questions ? / Vin...

Livre | Despret, Vinciane (1959-....) - philosophe. Auteur | 2012

Code de l'animal 2024 / [France]

Livre | Marguénaud, Jean-Pierre (1951-....). Directeur de publication | 2023 - 3e édition

Chargement des enrichissements...