0 avis
A mask-based diagnostic platform for point-of-care screening of Covid-19
Archive ouverte : Article de revue
International audience. Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200–500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL−1 in cultured SARS-CoV-2 suspensions. Using a “turn off” assay via ferrocenemethanol redox mediator, results about the infectivity state of the patient are obtained in 10 min.
Consulter en ligne
Suggestions
Du même auteur
Du même sujet
![Le système de santé français aujourd'hui : enjeux et défis / [Collège des économistes de la santé] | Barnay, Thomas (1976-....). Directeur de publication](https://covers.syracuse.cloud/Cover/ICTL/MONO/vRnxpu9w78wFRf2tlpZQgw2/9782747231855/MEDIUM?fallback=https%3a%2f%2fbibliotheque.univ-catholille.fr%2fui%2fskins%2fICTL%2fportal%2ffront%2fimages%2fGeneral%2fDocType%2fMONO_MEDIUM.png "Plus d'infos sur 'Le système de santé français aujourd'hui : enjeux et défis / [Collège des économistes de la santé]'")
![[Review] The role of the surface ligand on the performance of electrochemical SARS-CoV-2 antigen biosensors | Szunerits, Sabine](https://hal.archives-ouvertes.fr/hal-03335177/thumb/medium "Plus d'infos sur '[Review] The role of the surface ligand on the performance of electrochemical SARS-CoV-2 antigen biosensors'")
