Multivariate analysis of chemical and genetic diversity of wild Humulus lupulus L. (hop) collected in situ in northern France

Archive ouverte : Article de revue

Paguet, Anne-Sophie | Siah, Ali | Lefèvre, Gabriel | Moureu, Sophie | Cadalen, Thierry | Samaillie, Jennifer | Michels, Franck | Deracinois, Barbara | Flahaut, Christophe | Alves dos Santos, Harmony | Etienne-Debaecker, Audrey | Rambaud, Caroline | Chollet, Sylvie | Molinié, Roland | Fontaine, Jean-Xavier | Waterlot, Christophe | Fauconnier, Marie-Laure | Sahpaz, Sevser | Rivière, Céline

Edité par HAL CCSD

The hop plant (Humulus lupulus L.) has been exploited for a long time for both its brewing and medicinal uses, due in particular to its specific chemical composition. These last years, hop cultivation that was in decline has been experiencing a renewal for several reasons, such as a craze for strongly hopped aromatic beers. In this context, the present work aims at investigating the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. These wild hops were compared to ten commercial varieties and three heirloom varieties cultivated in the same sampled geographical area. Genetic analysis relying on genome fingerprinting using 11 microsatellite markers showed a high level of diversity. A total of 56 alleles were determined with an average of 10.9 alleles per locus and assessed a significant population structure (mean pairwise FST = 0.29). Phytochemical characterization of hops was based on volatile compound analysis by HS-SPME GC-MS, quantification of the main prenylated phenolic compounds by UHPLC-UV as well as untargeted metabolomics by UHPLC-HRMS and revealed a high level of chemical diversity among the assessed wild ac-cessions. In particular, analysis of volatile compounds revealed the presence of some minor but original com-pounds, such as aromadendrene, allo-aromadendrene, isoledene, fl-guaiene, alpha-ylangene and fl-pinene in some wild accessions; while analysis of phenolic compounds showed high content of fl-acids in these wild accessions, up to 2.37% of colupulone. Genetic diversity of wild hops previously observed was hence supported by their chemical diversity. Sample soil analysis was also performed to get a pedological classification of these different collection sites. Results of the multivariate statistical analysis suggest that wild hops constitute a huge pool of chemical and genetic diversity of this species.

Consulter en ligne

Suggestions

Du même auteur

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule | Bernard, Guillaume

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-...

Archive ouverte: Article de revue

Bernard, Guillaume | 2020-10

International audience. Cichorium intybus L. (Asteraceae) is an important industrial crop, as well as a medicinal plant which produces some bioactive compounds implicated in various biological effects with potential...

Anti-HCV Tannins From Plants Traditionally Used in West Africa and Extracted With Green Solvents | Bamba, Moussa

Anti-HCV Tannins From Plants Traditionally Used in West Africa and Extracte...

Archive ouverte: Article de revue

Bamba, Moussa | 2022-01-28

International audience. Millions of people are still infected with hepatitis C virus (HCV) nowadays. Although recent antivirals targeting HCV proteins are very efficient, they are not affordable for many people infe...

Chicory: Understanding the Effects and Effectors of This Functional Food | Pouille, Céline

Chicory: Understanding the Effects and Effectors of This Functional Food

Archive ouverte: Article de revue

Pouille, Céline | 2022-03

International audience. Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects. Whether it is the roasted root, the flour obtained from the roo...

Du même sujet

Can we Trust Untargeted Metabolomics: Results of the Metabo-ring Initiative, a Large-scale Multi-instruments Inter-laboratoire Study | Martin, Jean-Charles

Can we Trust Untargeted Metabolomics: Results of the Metabo-ring Initiative...

Archive ouverte: Article de revue

Martin, Jean-Charles | 2015

This article is published with open access at Springerlink.com. International audience. The metabo-ring initiative brought together five nuclear magnetic resonance instruments (NMR) and 11different mass spectrometer...

Analyse physique des sols : méthodes choisies / Clément Mathieu,... Françoise Pieltain,... | Mathieu, Clément (1940-....). Auteur

Analyse physique des sols : méthodes choisies / Clément Mathieu,... Françoi...

Livre | Mathieu, Clément (1940-....). Auteur | 1998

Analyse physique des sols est avant tout un manuel pratique décrivant des analyses et des mesures physiques des sols réalisées soit in situ, soit en laboratoire. Ces analyses et méthodes sont rassemblées en 7 grandes rubriques : -...

L'imprimerie, histoire et techniques / Guy Biart | Biart, Guy. Auteur

L'imprimerie, histoire et techniques / Guy Biart

Livre | Biart, Guy. Auteur | 1983

Du papier mécanique et de ses apprêts dans les diverses impressions / par Georges Olmer | Olmer, Georges. Auteur

Du papier mécanique et de ses apprêts dans les diverses impressions / par G...

Livre | Olmer, Georges. Auteur | 1882

"Ouvrage accompagné d'un tableau de concordance entre le poids des divers formats".

Sinicæ historiæ decas prima : res à gentis origine ad Christum natum in extrema Asia, sive magno Sinarum imperio gestas complexa / Martini Martinii | Martini, Martino (1614-1661). Auteur

Sinicæ historiæ decas prima : res à gentis origine ad Christum natum in ext...

Livre | Martini, Martino (1614-1661). Auteur | 1658

Opera historica omnia : Burgundica, Austriaca, Belgica: de rebus a principibus Burgundis atque Austriacis, qui Belgis imperarunt, pace belloque praeclare gestis. Insertus est ejusdem de veterstate et nobilitate familiae Halsburgicae et Austriacae liber singularis. Acce sereque de veterum ac sui saeculi Belgio libri duo, alique / [by I. Coppenii, ed.] / Pontus Heuterus | Heuterus, Pontus (1535-1602). Auteur

Opera historica omnia : Burgundica, Austriaca, Belgica: de rebus a principi...

Livre | Heuterus, Pontus (1535-1602). Auteur | 1649 - Nunc primum simul edita, opera atique industria viri docti recensita, & catibus distincta.

Chargement des enrichissements...