0 avis
A multi-center peptidomic investigation of simulated gastrointestinal food digestion
Archive ouverte : Communication dans un congrès
Edité par HAL CCSD
This conference is a major event in the field of Food, Nutrition and Health. It is organized in the frame of the INFOGEST research network(www.cost-infogest.eu) whose objective is to “improve the health properties of food by sharing our knowledge on the digestive process”. INFOGESTis a global network of approximately 582 research scientists (academic and food companies) from 150 institutions across 46 countries.. International audience. Mass spectrometry has become the technique of choice for the simultaneous assessment of a high variety of molecules in complex food matrices. For this reason, it is best suited for precisely monitoring the evolution of digestive processes in vivo and in vitro. However, due to the variety of equipment available in different laboratories as well as settings, statistical evaluations, and interpretations, it is difficult to predict a priori the ideal parametersfor best results. Methods: The present work addressed that gap by executing an inter-laboratory study with samples collected during in vitro digestion and presenting an overview of the state of the art mass spectrometry applications and analytical capabilities available for studying food digestion.Results: Three representative high-protein foods, skim milk powder, chicken breast and tofu, were digested according to the INFOGEST protocol with sample collection at five time points during gastric and intestinal digestion. Nine laboratories analyzed all digesta with their in house equipment, conventional sample treatment procedure, data evaluation methods, and graphical representation tools. Conclusions: The compiled results demonstrate a strong consensus among labs in terms of major protein degradation for the three foods matrices and present suitable methodical andstatistical approaches for representing the analytical results.
Consulter en ligne
Suggestions
Du même auteur
