Comparison of paromomycin efficiency on the Cryptosporidium parvum development by standard methods and impedance spectroscopy

Archive ouverte : Poster de conférence

Leniere, Anne-Charlotte | Follet, Jérôme | Vlandas, Alexis

Edité par HAL CCSD

P-34, Poster Session 1. International audience. Cryptosporidiosis is an important zoonosis worldwide, causing intestinal infection to humans and a wide range of animals. Cryptosporidium parvum is one of the major causes of neonatal calf diarrhea leading to economic losses and compromised animal welfare. Despite the high prevalence, no vaccine or drug therapy is available yet. Research for new drugs against Cryptosporidium met difficulties due to its obligate intracellular parasite status requiring the support of a host cell and the absence of standardized/automated tools enabling the study of the parasite and drug screening. In this study Human ileocecal colorectal adenocarcinoma cells (HCT-8) were grown to confluency and infected by C. parvum during 48 h with a range of paromomycin concentrations, the gold standard for in vitro screening. Results were obtained by standard methods such as real-time qPCR and microscopy. The level of infection was assessed using fluorescent images combined with an automatic detection of parasites using a Fiji plugin and morphometric data for each parasite were recorded. The half maximal inhibitory concentrations (IC50) of paromomycin were obtained by both methods and will be compared to a new approach using an electrical impedance-based device to quantify infectivity of HCT-8 infected by C. parvum and for drug screening assessment. First results with the impedance signal shown a reproductible peak at 12 h post-infection which could be used as an infectivity sensor, faster than current methods.

• Contient
• Sujet
• Description
• Oeuvre